Journal: BMC oral health
Article Title: Preparation and characterization of bovine dental pulp-derived extracellular matrix hydrogel for regenerative endodontic applications: an in vitro study.
doi: 10.1186/s12903-024-05004-z
Figure Lengend Snippet: Fig. 6 Growth factor content; (a) Comparison between P-ECM & P-ECM + HA hydrogels regarding TGF-β1 release showing increased release in P-ECM + HA at 0,14 and 28 days ; (b) bFGF release showing higher release in P-ECM + HA group in 1,5 and 14 days ; (c) BMP2 release showing nearly indetectable concentration in P -ECM + HA while detectable in P-ECM; (d) VEGF release showing increased release of P-ECM at 7 days timepoint (e, f, g&h) bar graph showing comparison between P-ECM pre-gel and zero time point hydrogel after gelation
Article Snippet: The levels of TGF-β1, bFGF, BMP-2, and VEGF growth factors were assayed in supernatants of P-ECM and P-ECM + HA hydrogels and in pre-gel of P-ECM, using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions [human bone morphogenetic protein 2 (BMP-2) immunoassay, R&D Systems Inc, USA]; [Transforming growth factor beta 1 (TGF-β1 ) immunoassay, Cloud Clones Corp, USA]; [ bovine basic fibroblast growth factor (bFGF) and bovine vascular endothelial growth factor (VEGF), BTlab, China].
Techniques: Comparison, Concentration Assay